Thermal hysteresis measurement Sakae Tsuda
 
 
   

Instruments

Linkam LTS120

Ice bipyramid

    
(1/4) Here I introduce a protocol to measure the thermal hysteresis (TH) activity of an AFP sample. The Nanoliter osmometer would be a standard instrument developed for such purpose. We while utilize an ordinary photomicrosystem equipped with Linkam LTS120 (UK) temperature control unit that can lower the sample temperature to -40 degC with a Peltier device. First we need to freeze entirely the AFP sample in a capillary tube [1] by lowering of the temperature to -40 degC. Please watch the movie 1 showing how the sample is frozen. (->next) (->home)